TruNeo: an integrated pipeline improves personalized true tumor neoantigen identification

摘要

Overview of TruNeo neoantigen prediction pipeline from next-sequencing data to candidate peptides. Flowchart shows the computing steps (trapezoidal box) and corresponding input/result (square box) of TruNeo pipeline. The pipeline includes: (1) Alteration identification, including missense mutations, InDels and gene fusions; (2) HLA typing; (3) gene expression quantification; (4) neo-peptide prediction; (5) MHC I binding affinity prediction for selecting candidate neo-peptide; (6) neo-peptide ranking according to multiple biological processes