Schematic representation of LysKB317 domain structures and putative endolysin catalytic site on peptidoglycan of L. fermentum. a The LysKB317 is a recombinant (912 bp) 6 × His-tag phage lytic protein (33.8 kDa). The endolysin architecture (not to scale) consist of a fused N-terminal 6 × His-tag, glycosidase family 25 (GH25) lysin A-like enzymatic activity domain and a cell wall binding SH3b homologue domain based on amino acid homologies and conserved domains (catalytic and cell-wall binding) prediction data base. (GenBank accession number {"type":"entrez-protein","attrs":{"text":"AIY32273.1","term_id":"725797515","term_text":"AIY32273.1"}}AIY32273.1). b The predicted LysKB317 catalytic site against Lactobacillus fermentum repeated peptidoglycan structure (modified from [4, 25, 26]) based on amino acid homologies to other biochemically characterized lytic enzymes [36]. D-Asx stands for D-Asp or amidated D-Asp (D-Asn [53])
展开▼
