首页> 美国卫生研究院文献>Ecology and Evolution >A molecular phylogeny of historical and contemporary specimens of an under‐studied micro‐invertebrate group
【2h】

A molecular phylogeny of historical and contemporary specimens of an under‐studied micro‐invertebrate group

机译:历史和当代标本的分子系统的历史和当代标本进行了审查的微型无脊椎动物组

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Resolution of relationships at lower taxonomic levels is crucial for answering many evolutionary questions, and as such, sufficiently varied species representation is vital. This latter goal is not always achievable with relatively fresh samples. To alleviate the difficulties in procuring rarer taxa, we have seen increasing utilization of historical specimens in building molecular phylogenies using high throughput sequencing. This effort, however, has mainly focused on large‐bodied or well‐studied groups, with small‐bodied and under‐studied taxa under‐prioritized. Here, we utilize both historical and contemporary specimens, to increase the resolution of phylogenetic relationships among a group of under‐studied and small‐bodied metazoans, namely, cheilostome bryozoans. In this study, we pioneer the sequencing of air‐dried cheilostomes, utilizing a recently developed library preparation method for low DNA input. We evaluate a de novo mitogenome assembly and two iterative methods, using the sequenced target specimen as a reference for mapping, for our sequences. In doing so, we present mitochondrial and ribosomal RNA sequences of 43 cheilostomes representing 37 species, including 14 from historical samples ranging from 50 to 149 years old. The inferred phylogenetic relationships of these samples, analyzed together with publicly available sequence data, are shown in a statistically well‐supported 65 taxa and 17 genes cheilostome tree, which is also the most broadly sampled and largest to date. The robust phylogenetic placement of historical samples whose contemporary conspecifics and/or congenerics have been sequenced verifies the appropriateness of our workflow and gives confidence in the phylogenetic placement of those historical samples for which there are no close relatives sequenced. The success of our workflow is highlighted by the circularization of a total of 27 mitogenomes, seven from historical cheilostome samples. Our study highlights the potential of utilizing DNA from micro‐invertebrate specimens stored in natural history collections for resolving phylogenetic relationships among species.
机译:在较低的分类水平下的关系的解决对于回答许多进化问题至关重要,因此,充分不同的物种表示是至关重要的。后一种目标并不总是可以使用相对新鲜的样品来实现。为了缓解采购雷达的困难,我们已经看到,使用高通量测序的建立分子系统中的历史标本的利用率越来越多。然而,这种努力主要集中在大型或良好的群体上,具有小于身体的小型和介绍的征集。在这里,我们利用历史和当代标本,增加一组医学和小型美唑烷的系统发育关系的分辨率,即杂铜组合。在这项研究中,我们利用最近开发的文库制备方法对低DNA投入进行了萌发的风干乳酸晶体的测序。我们使用测序的目标样本作为用于测绘的参考,评估DE Novo促催化组件和两种迭代方法。在这样做时,我们呈现出代表37种的33个乳霜菌的线粒体和核糖体RNA序列,其中包括来自50至149岁的历史样品。这些样品的推断系统发育关系与可公同可用的序列数据分析,在统计上良好地支持的65个征征的65个分类群和17个基因芝麻骨树中,这也是最广泛的采样和最大的迄今为止。已经测序的历史样本的强大系统发育放置,其当代消费和/或同胞学进行了测序,验证了我们工作流程的适当性,并给予那些历史样本的系统发育放置的置信度,其历史样本没有测序。我们的工作流程的成功被总共27例诱导症的循环化突出显示,七种遗迹杂志样本。我们的研究突出了利用储存在自然历史收集中的微型无脊椎动物标本中DNA的潜力,以解决物种之间的系统发育关系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号