Exosomal lncRNA DOCK9-AS2 derived from cancer stem cell-like cells activated Wnt/β-catenin pathway to aggravate stemness proliferation migration and invasion in papillary thyroid carcinoma

摘要

a Venn diagram showed 36 lncRNAs with significant upregulation in PTC samples in the intersection of predicted results from circlncRNAnet and GEPIA2. b 36 candidate lncRNAs were analyzed in PTC tissues (n = 3) by RT-qPCR, versus the matched para-tumorous tissues (n = 3). c RT-qPCR of DOCK9-AS2 level in PTC tumor samples (n = 54) versus paired para-tumorous samples (n = 54). d RT-qPCR data of DOCK9-AS2 level in PTC patients at stage III/IV (n = 33) versus stage I/II (n = 21) and in metastatic patients (n = 30) versus non-metastatic patients (n = 24). e RT-qPCR analysis for the DOCK9-AS2 level in the plasma exosomes from PTC cases (n = 54) healthy control (n = 44). f. RT-qPCR of DOCK9-AS2 expression in PTC cell lines (TPC1 and BAPAB) compared with normal cell line (Nthy-ori3-1). g RT-qPCR detection of DOCK9-AS2 level in exosomes from PTC cells versus normal Nthy-ori3-1 cells. h FISH images of DOCK9-AS2 in PTC cells. Scale Bar: 10 μm. i Subcellular fractionation for DOCK9-AS2 expression in cytoplasm and nucleus of PTC cells. Scale bar: 10 μm. GAPDH and U6 were cytoplasmic and nuclear references. **P < 0.01. Error bar denotes Mean ± S.D.