Exogenous NADPH ameliorates myocardial ischemia–reperfusion injury in rats through activating AMPK/mTOR pathway

摘要

NADPH reduced myocardial I/R-induced injury in rats. Myocardial I/R was established by ligating the left anterior descending artery in rats. NADPH 4, 8, and 16 mg/kg (N4, N8, and N16) and diltiazem were administered intravenously at the onset of reperfusion. The infarct size was evaluated 2 h after reperfusion. Representative photographs (a) and quantitative analysis (b) showed that NADPH decreased the infarct size. AAR area at risk, IS infarct size, LV left ventricle. c Cardiac function was evaluated by serum LDH at 2 h and cTn-I at 4 h after reperfusion. LDH lactate dehydrogenase, cTn-I cardiac troponin I. n = 6~8 rats. The bars represent the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the I/R group