Transcriptomic analysis of the mechanisms of alleviating renal interstitial fibrosis using the traditional Chinese medicine Kangxianling in a rat model

摘要

Experimental design and analytical procedures. SD rats were divided into a control group and a RIF model group. The model rats were further divided into 5 groups (n = 5 per group): model validation group, OC group (RIF sham control), HK group (high concentration of KXL), LK group (low concentration of KXL) and LOS group (losartan treatment). The model validation group rats were subjected to renal histopathological section staining. The NC group (sham control) received sham and empty vehicle treatment. Histological analyses validated the establishment of RIF modelling and the treatment effect of KXL. Multi-level transcriptomic datasets were generated using lncRNA, mRNA and microRNA sequencing of kidney tissues. Functional annotations and pathway analyses were applied to unravel the therapeutic mechanisms. A multi-level transcriptomic regulatory network was built to illustrate the core factors in fibrosis pathogenesis and therapeutic regulation.