Enterovirus A71 capsid protein VP1 increases blood–brain barrier permeability and virus receptor vimentin on the brain endothelial cells

摘要

VP1 increased BBB permeability in vivo. Western blotting showed the increased albumin in the brain tissues from the mice treated with VP1. Immunohistochemistry staining revealed evident albumin in the brain tissues from the mice treated with VP1. , Fluorescence intensity of Evans blue dye (EBD) or FD4 was significantly elevated in the brain tissues from the mice treated with VP1. Expression levels of tight junction proteins in the brain tissues. ZO-1 was comparable in the mice treated with normal saline or VP1. Claudin-5 was significantly reduced in the brain tissues treated with VP1. *