Chemical contrast for imaging living systems: molecular vibrations drive CARS microscopy

摘要

( ) Schematic diagram of the transitions involved for Raman spectroscopy (top) and CARS spectroscopy (bottom). Both the pump beam and the Stokes beam are required to obtain a CARS signal. ( ) Schematic illustration showing linear Raman scattering of the probe beam (left) and nonlinear coherent anti-Stokes Raman scattering (right). ( ) Raman spectra corresponding to various cellular components.