Skeletal muscle targeting in vivo electroporation-mediated HGF gene therapy of bleomycin-induced pulmonary fibrosis in mice

摘要

( ) Procedure of the skeletal muscle-targeting electroporation following intramuscular injection of plasmid DNA. ( ) Expression of GFP in the quadriceps muscle at day 1 ( ), 5 ( ), 7 ( ), and 28 ( ). Panel represents the number of GFP positive mice. Asterisk ( ) indicates the data from bleomycin-untreated mice. ( and ) Concentration of human HGF in the unilateral quadriceps muscle ( ) or lung ( ) at days 1, 3, 5, 7, 14, and 28; (•) human HGF level of mice transferred with pUC-HGF; (○) human HGF level of mice transferred with pCAGGS-EGFP. Each plot represents the mean value from six mice.