Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

摘要

A total of 123 strains were isolated from different habitats and tested for their ability to degrade cellulose and xylan by simple plate screening method. Among strains, more than 34 and 45% respectively, exhibited higher cellulolytic and xylanolytic activity, compared to the reference strain QM 9414. For strains efficiently degrading cellulose, a highest enzyme activity was confirmed using filter paper test, and it resulted in a range from 1.01 to 7.15 FPU/ml. Based on morphological and molecular analysis, the isolates were identified as . The most frequently identified strains belonged to species. Among all strains, the most effective in degradation of cellulose and xylose was and , especially those isolated from forest wood, forest soil or garden and mushroom compost. The results of this work confirmed that numerous strains from the species have high cellulose and xylan degradation potential and could be useful for lignocellulose biomass conversion . for biofuel production.