Tools for Mos1-mediated single copy insertion (mosSCI) with excisable unc-119(+) or NeoR (G418) selection cassettes

摘要

Overview of plasmids for mosSCI at -compatible sites using G418 ( ) (A) or (+) (B) selection cassettes flanked by loxP sites. Both plasmids are modified from pCFJ151 (ttTi5605-MCS) (Frokjaer-Jensen 2008). Results of test injections (three different transgene constructs for each plasmid) are shown below the diagrams. The mosSCI “direct injection protocol” was followed, using P and three (+) co-injection markers to screen against extrachromosomal arrays as described (Frokjaer-Jensen 2012)(www.wormbuilder.org). Transgenes in pALS297/ or pALS186/ were used for mosSCI injections into strains ALS344 or EG6699, respectively (Table 1).