Focus: Skin: In vitro Reducing Effect of Cloxacillin on Minimum Inhibitory Concentrations to Imipenem Meropenem Ceftazidime and Cefepime in Carbapenem-resistant Pseudomonas aeruginosa Isolates

摘要

Today, resistance to antibacterial agents is the most important problem facing public health. is a common gram-negative bacterium and an important cause of nosocomial infections. Resistance to many antibiotics in strains of isolated from hospital settings such as cephalosporins and carbapenems have been recently reported. Therefore, the introduction of a new strategy to treat the infection of these organisms will be beneficial. In this study we determined the ability of cloxacillin to reduce Minimum Inhibitory Concentrations (MICs) of carbapenem-resistant to imipenem (IMI), meropenem (MEM), ceftazidime (CAZ), and cefepime (FEP). From 2015 to 2017, 61 non-duplicates of carbapenem-resistant were collected from clinical samples of hospitalized patients in Kerman, Iran. The MICs of the isolates to IMI, MEM, CAZ, and FEP with/without cloxacillin were determined by microbroth dilution method. The level of MIC of isolates to carbapenems (IMI and MEM) and cephalosporins (CAZ and FEP) ranged from 1-256 μg/mL and 4-1024 μg/mL alone and from 1-32 μg/mL and 1-512 μg/mL in combination with cloxacillin, respectively. The MIC showed a significant difference reduction after the addition of cloxacillin (P ≤ 0.05). Our results showed potentially of cloxacillin in reduction of MIC to IMI, MEM, CAZ, and FEP in multi-drug resistant therefore combination of these antibiotics with cloxacillin could be beneficial for treatment of infections caused by multi-drug resistant