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A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

机译:研究鸡血藤木质部和韧皮部代谢产物分布规律的方法

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摘要

Spatholobi Caulis (SC), the vine stem of Dunn, is a widely used traditional Chinese medicine (TCM) for the treatment of blood stasis syndrome and related diseases. Xylem and phloem are the main structures of SC and the color of xylem in SC is red brown or brown while the phloem with resin secretions is reddish brown to dark brown. They are alternately arranged in a plurality of concentric or eccentric rings. In order to investigate the distribution patterns of metabolites in xylem and phloem of SC, an analytical method based on UFLC–QTRAP–MS/MS was established for simultaneous determination of 22 constituents including four flavanols, nine isoflavones, two flavonols, two dihydroflavones, one flavanonol, one chalcone, one pterocarpan, one anthocyanidin and one phenolic acid in the samples (xylem and phloem) from Laos. Furthermore, according to the contents of 22 constituents, heat map, principal components analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS–DA) and t–test were used to evaluate the samples and discover the differences between xylem and phloem of SC. The results indicated that the measured ingredients in xylem and phloem were significantly different. To be specific, the contents of flavonoids in xylem were higher than that in phloem, while the content of protocatechuic acid showed a contrary tendency. This study will not only reveal the distribution patterns of metabolites in xylem and phloem of SC but also facilitate further study on their quality formation.
机译:邓恩的葡萄干Spatholobi Caulis(SC)是广泛用于治疗血瘀证和相关疾病的中药(TCM)。木质部和韧皮部是SC的主要结构,SC中木质部的颜色是红棕色或棕色,而带有树脂分泌物的韧皮部是红棕色至深棕色。它们交替地布置在多个同心或偏心环中。为了研究SC木质部和韧皮部中代谢物的分布方式,建立了一种基于UFLC-QTRAP-MS / MS的分析方法,用于同时测定22种成分,其中包括4种黄烷醇,9种异黄酮,2种黄酮醇,2种二氢黄酮,1种。老挝样品(木质部和韧皮部)中的黄烷醇,一种查尔酮,一种紫果胶,一种花青素和一种酚酸。此外,根据22种成分的含量,用热图,主成分分析(PCA),正交偏最小二乘判别分析(OPLS-DA)和t检验对样品进行评估,发现木质部和韧皮部的差异。 SC。结果表明,测得的木质部和韧皮部成分存在显着差异。具体而言,木质部中的类黄酮含量高于韧皮部中的含量,而原儿茶酸的含量呈现相反的趋势。这项研究不仅揭示了SC中木质部和韧皮部代谢产物的分布方式,而且还有助于进一步研究其质量形成。

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