Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities

摘要

Indirect open-circuit calorimetry in gnotobiotic isolators. Schematic drawing of the experimental apparatus. Two isolators are shown: isolator A contains animals colonized with bacterial consortium A from the lean co-twin; isolator B contains animals harboring bacterial consortium B from the obese co-twin. Photograph of flexible film gnotobiotic isolator containing a metabolic chamber and devices used to connect the chamber to the exterior calorimeter