Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis

摘要

Isolation and characterization of white and brown adipocytes. White fat and brown adipose tissue, left; cultured white and brown adipocytes, right. Results of RT-qPCR on marker genes of white and brown adipocytes. White-fat marker gene, left; brown-fat marker gene, right. Western blots and analysis of gray of white and brown adipocytes in C57BL/6 mice. Immunofluorescence staining of GAPDH (control), LEPTIN, and UCP1 in white and brown adipocytes. Bar represents 100 μm. Morphological observation and Oil Red-O staining of adipogenic differentiation