Transforming growth factor-beta1 promotes Geistlich Bio-Oss® osteogenesis via inhibiting local inflammation response in vivo

摘要

Inhibiting inflammation is helpful in relieving the absorption of alveolar bone and promoting periodontal bone regeneration. In a previous study, we showed that transforming growth factor-beta1 (TGF-β1)-induced Treg cells inhibit the absorption of tissue-engineered cartilage caused by endogenous IFN-γ and TNF-α. In this study, we investigated the effect of inhibiting local inflammatory responses on Geistlich Bio-Oss osteogenesis promotion in vivo. TGF-β1 BMMSCs (bone marrow mesenchymal stem cells) were cultured in Geistlich Bio-Oss medium, and biocompatibility was evaluated. Alveolar bone defects in New Zealand rabbits repaired by application of Geistlich Bio-Oss were compared to the effects of added TGF-β1 BMMSCs. There was no significant difference between the untreated Geistlich Bio-Oss medium-control group and the group treated with the addition of TGF-β1 BMMSCs. Pro-inflammatory cytokines IFN-γ and TNF-α delayed Geistlich Bio-Oss -induced osteogenesis, but no significant difference in osteogenesis was seen with the addition of TGF-β1 BMMSCs. Geistlich Bio-Oss has good compatibility with TGF-β1 BMMSCs. However, the dual role of TGF-β1 BMMSCs in regenerating periodontal bone and limiting local inflammation is not clear.