A Novel LC-MS/MS Assay for Vitamin B1 B2 and B6 Determination in Driedblood Spots and Its Application in Children

摘要

Several HPLC assays can be used to determine vitamin B1 (thiamin), B2 (riboflavin) and B6 (PLP) in biological matrix. However, no existing LC-MS/MS methods have been developed for quantifying thiamin, riboflavin and PLP in dried blood spots (DBSs), which are often used as a sampling/storage vessel for finger blood from infants and children. This study firstly used vacuum package in DBS cards storage, and evaluated the validity, reliability and stability of an LC-MS/MS assay for measuring thiamin, riboflavin and PLP in DBS cards.