IL-33-induced neutrophil extracellular traps degrade fibronectin in a murine model of bronchopulmonary dysplasia

摘要

E14–15d pregnant dams were intraperitoneally injected with 75 µg/kg LPS, and newborn mice were exposed to 65% hyper-oxygen to establish BPD model. – Lung morphometry was analyzed by H&E staining of tissue samples from surviving pups at day 3, 7, 14, and 28 post birth. Scale bars = 200 μm. Representative immunohistochemical analysis of fibronectin in pulmonary tissues at day 14 post birth. Scale bars, 100 μm. – Western blotting analysis of fibronectin expression in pulmonary tissues at day 14 and 28 post birth. IL-1β, IL-6, TNF-α, and IL-33 mRNA expression in pulmonary tissues at day 14 post birth. – IL-33 protein expression in pulmonary tissues at day 14 and 28 post birth. Three to four mice were included in the control and BPD group at each time point. All experiments were repeated at least three times. *  P P