Integrated bioinformatics analysis of aberrantly-methylated differentially-expressed genes and pathways in age-related macular degeneration

摘要

The flowchart of this study. The data of expression profiling and methylation profiling were obtained from Gene Expression Omnibus (GEO) database. As a result, we identified 827 high-expression genes and 592 low expression genes; 4117 hypermethylated genes and 511 hypomethylated genes. To further explore the aberrantly methylated differentially expressed genes, Hypo-HGs were obtained by overlapping hypomethylation and up-regulated genes; Hyper-LGs were obtained by overlapping hypermethylation and down-regulated genes. In total, we identified 24 Hypo-HGs and 153 Hyper-LGs. Then, functional enrichment analysis and protein–protein interaction (PPI) network analysis of screened genes were performed