BCREval: a computational method to estimate the bisulfite conversion ratio in WGBS

摘要

The characteristics of bisulfite converted telomeric WGSBS data. The distribution of read with distinct number of telomeric blocks in two sample (ENCLB443JJF and ENCLB890RFU). The G-strand and C-strand original reads are color blue and orange respectively. FASTQ1 and FASTQ2 indicate the file containing forward and reversed reads in paired-end NGS sequencing; A scatter plot showing the numbers of telomeric reads and total reads of 12 WGSBS experiments (red and orange dots represent tissue samples); UCRs calculated by using forward (+) and reversed (-) reads in three cytosines sites in two samples (ENCLB098BGY, ENCLB167QQW); The UCRs of technical repeats of two tissues (skeletal muscle myoblast and mouse liver) and two cell lines (HepG2 and H1-hESC); The box-plot showing the distribution of UCR of three cytosines sites across eleven samples.