Prediction model construction of mouse stem cell pluripotency using CpG and non-CpG DNA methylation markers

摘要

Overview of epigenetic and transcriptomic analysis of single stem cells for pluripotency prediction. In total, 75 mouse single embryonic stem cells ( ), for which gene expression and DNA methylation were sequenced simultaneously, were used to generate a linear model that predicts cell pluripotency. The biological pseudo-time of each cell was determined using gene expression data. Using the CpG and non-CpG DNA methylation levels of each genomic interval, we constructed a linear model that predicts cell pseudo-time. Validation was performed using DNA methylation sequencing data from 34 independent samples of single and bulk ESCs ( )