Coherent anti-Stokes Raman scattering (CARS) microscopy is emerging as a powerful method for imaging materials and biological systems, partly because of its noninvasiveness and selective chemical sensitivity. However, its full potential for species-selective imaging is limited by a restricted spectral bandwidth. Recent increases in bandwidth are promising but still are not sufficient for the level of robust component discrimination that would be needed in a chemically complex milieu found, for example, in intracellular and extracellular environments. We demonstrate a truly broadband CARS imaging instrument that we use to acquire hyper-spectral images with vibrational spectra over a bandwidth of 2500 cm−1 with a resolution of 13 cm−1.
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机译:相干抗斯托克斯拉曼散射(CARS)显微镜正在成为一种使材料和生物系统成像的强大方法,部分原因是其无创性和选择性的化学敏感性。但是,其对物种选择性成像的全部潜力受到光谱带宽的限制。最近带宽的增加是有希望的,但是对于在例如细胞内和细胞外环境中发现的化学复杂环境中所需的鲁棒的组分辨别水平还不够。我们演示了一款真正的宽带CARS成像仪器,该仪器可用于在2500 cm -1 sup>的带宽上以13 cm -1 sup>的分辨率采集具有振动光谱的高光谱图像。
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