18Ffallypride dopamine D2 receptor studies using delayed microPET scans and a modified Logan plot

摘要

[¹⁸F]fallypride PET studies can be used to estimate the non-displaceable binding potential (BPND) in vivo of dopamine D2/D3 receptor-rich regions of the brain. These studies often take considerable time, up to two or more hours, limiting the throughput. In this work, we investigated whether limited-duration scans performed subsequent to tracer administration yielded stable BPND estimates. In particular, we applied a modified version of the Logan plot method on the last 60 min of 120 min data and compared the results to those from analysis of the full data set.MethodsFourteen male Sprague-Dawley rats were injected with [¹⁸F]fallypride intravenously while under isoflurane anesthesia and dynamic data were acquired on the microPET Focus 220 for 120 min. The distribution volume ratio (DVR = BPND + 1) was calculated from a Logan plot using 120 min of data and from a modified version using only the last 60 min. Three of these rats were imaged again on a second day to test the reproducibility. A two-tissue compartment model also was used to fit the time activity curves (TACs) of the 120 min scans to estimate the parameters K1, k2, kon, k4, and Bmax. These parameters then were used to simulate similar TACs while changing kon to reflect changes in the dopaminergic system. The simulated TACs were used as a means for exploring the differences in DVR estimates between the last 60 min only and the full 120 min of simulated data.