首页> 美国卫生研究院文献>other >A Genome-Wide siRNA Screen to Identify Host Factors Necessary for Growth of the Parasite Toxoplasma gondii
【2h】

A Genome-Wide siRNA Screen to Identify Host Factors Necessary for Growth of the Parasite Toxoplasma gondii

机译:全基因组siRNA筛选以鉴定寄生虫弓形虫生长所需的宿主因子

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Toxoplasma gondii is an obligate intracellular parasite that is able to infect virtually any nucleated cell of all warm-blooded animals. The host cell factors important for parasite attachment, invasion, and replication are poorly understood. We screened a siRNA library targeting 18,200 individual human genes in order to identify host proteins with a role in T. gondii growth. Our screen identified 19 genes whose inhibition by siRNA consistently and significantly lowered parasite replication. The gene ontology categories for those 19 genes represented a wide variety of functions with several genes implicated in regulation of the cell cycle, ion channels and receptors, G-protein coupled receptors, and cytoskeletal structure as well as genes involved in transcription, translation and protein degradation. Further investigation of 5 of the 19 genes demonstrated that the primary reason for the reduction in parasite growth was death of the host cell. Our results suggest that once T. gondii has invaded and established an infection, global changes in the host cell may be necessary to reduce parasite replication. While siRNA screens have been used, albeit rarely, in other parasite systems, this is the first report to describe a high-throughput siRNA screen for host proteins that affect T. gondii replication.
机译:弓形虫是专性的细胞内寄生虫,几乎可以感染所有温血动物的任何有核细胞。对寄生虫附着,入侵和复制重要的宿主细胞因素了解甚少。我们筛选了针对18,200个单个人类基因的siRNA文库,以鉴定在弓形虫生长中起作用的宿主蛋白。我们的筛选确定了19个基因,这些基因的siRNA抑制作用始终如一且显着降低了寄生虫复制。这19个基因的基因本体论类别代表了各种各样的功能,其中几个基因与细胞周期,离子通道和受体,G蛋白偶联受体和细胞骨架结构的调控有关,以及与转录,翻译和蛋白质有关的基因降解。对19个基因中的5个的进一步研究表明,寄生虫生长减少的主要原因是宿主细胞的死亡。我们的研究结果表明,刚地弓形虫入侵并确定感染后,宿主细胞的整体变化可能对于减少寄生虫复制是必要的。尽管已经在其他寄生虫系统中使用了siRNA筛选,尽管很少使用,但这是第一份描述高通量siRNA筛选的影响宿主弓形虫复制的宿主蛋白的报告。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号