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The Design of Simple Bacterial Microarrays: Development towards Immobilizing Single Living Bacteria on Predefined Micro-Sized Spots on Patterned Surfaces

机译:简单细菌微阵列的设计:将单一活菌固定在带图案的表面上预先定义的微型斑点上的发展

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摘要

In this paper we demonstrate a procedure for preparing bacterial arrays that is fast, easy, and applicable in a standard molecular biology laboratory. Microcontact printing is used to deposit chemicals promoting bacterial adherence in predefined positions on glass surfaces coated with polymers known for their resistance to bacterial adhesion. Highly ordered arrays of immobilized bacteria were obtained using microcontact printed islands of polydopamine (PD) on glass surfaces coated with the antiadhesive polymer polyethylene glycol (PEG). On such PEG-coated glass surfaces, bacteria were attached to 97 to 100% of the PD islands, 21 to 62% of which were occupied by a single bacterium. A viability test revealed that 99% of the bacteria were alive following immobilization onto patterned surfaces. Time series imaging of bacteria on such arrays revealed that the attached bacteria both divided and expressed green fluorescent protein, both of which indicates that this method of patterning of bacteria is a suitable method for single-cell analysis.
机译:在本文中,我们演示了一种快速,简便且适用于标准分子生物学实验室的细菌阵列制备方法。微接触印刷用于在玻璃表面上的预定位置上沉积促进细菌附着的化学物质,该玻璃表面涂有以其对细菌附着的抵抗力而闻名的聚合物。使用聚多巴胺(PD)的微接触印刷岛在涂覆有抗粘剂聚合物聚乙二醇(PEG)的玻璃表面上获得了高度固定的细菌阵列。在这种涂有PEG的玻璃表面上,细菌附着在97%至100%的PD岛上,其中21%至62%被单个细菌占据。可行性测试显示,固定在带图案的表面上后,有99%的细菌存活。在这种阵列上对细菌进行时间序列成像显示,附着的细菌既分裂又表达绿色荧光蛋白,这两者都表明这种细菌构图方法是用于单细胞分析的合适方法。

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