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Comparative Study on Gluten Protein Composition of Ancient (Einkorn Emmer and Spelt) and Modern Wheat Species (Durum and Common Wheat)

机译:古代(EinkornEmmer和Spelt)和现代小麦(硬粒小麦和普通小麦)的麸质蛋白组成比较研究

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摘要

The spectrophotometric Bradford assay was adapted for the analysis of gluten protein contents (gliadins and glutenins) of spelt, durum wheat, emmer and einkorn. The assay was applied to a set of 300 samples, including 15 cultivars each of common wheat, spelt, durum wheat, emmer and einkorn cultivated at four locations in Germany in the same year. The total protein content was equally influenced by location and wheat species, however, gliadin, glutenin and gluten contents were influenced more strongly by wheat species than location. Einkorn, emmer and spelt had higher protein and gluten contents than common wheat at all four locations. However, common wheat had higher glutenin contents than einkorn, emmer and spelt resulting in increasing ratios of gliadins to glutenins from common wheat (< 3.8) to spelt, emmer and einkorn (up to 12.1). With the knowledge that glutenin contents are suitable predictors for high baking volume, cultivars of einkorn, emmer and spelt with good predicted baking performance were identified. Finally, spelt, emmer and einkorn were found to have a higher nitrogen partial factor productivity than common and durum wheat making them promising crops for a more sustainable agriculture.
机译:Bradford分光光度法适用于分析斯佩尔特,硬粒小麦,Emmer和einkorn的面筋蛋白含量(麦醇溶蛋白和谷蛋白)。该测定法应用于一组300个样品,其中包括15个品种,分别是同一年在德国四个地方种植的普通小麦,斯佩尔特小麦,硬质小麦,emmer和einkorn。总蛋白含量同样受位置和小麦种类的影响,但是麦醇溶蛋白,谷蛋白和面筋含量受小麦种类的影响远大于位置。在所有四个地方,Einkorn,emmer和pelled的蛋白质和面筋含量均高于普通小麦。然而,普通小麦的谷蛋白含量高于单粒小麦,单粒小麦和散粒小麦,导致普通小麦(<3.8)与拼花,单粒小麦和单粒小麦(最高12.1)的麦醇溶蛋白和麸质比例增加。知道谷蛋白含量是高烘焙量的合适预测因子,因此鉴定出了具有良好预测烘焙性能的einkorn,emmer和拼写品种。最后,发现斯佩尔特,埃默恩和英肯恩比普通小麦和硬质小麦具有更高的氮分因子生产率,这使它们有希望成为更可持续农业的作物。

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