RNase H2-Dependent Polymerase Chain Reaction and Elimination of Confoundersin Sample Collection Storage and Analysis Strengthen Evidence That microRNAs in BovineMilk Are Bioavailable in Humans

摘要

BackgroundEvidence suggests that dietary microRNAs (miRs) are bioavailable and regulate gene expression across species boundaries. Concerns were raised that the detection of dietary miRs in plasma might have been due to sample contamination or lack of assay specificity. >Objectives: The objectives of this study were to assess potential confounders of plasma miR analysis and to detect miRs from bovine milk in human plasma.