BackgroundVisceral hypersensitivity (VH) is a common condition in many gastrointestinal disorders such as inflammatory bowel diseases (IBDs) in human and animals. Most studies often induce Crohn’s disease/colitis to investigate VH in small experimental animals. Although farm animals commonly suffer from IBDs, their VH has not been investigated so far. Because goats can suffer from Johne’s disease, a naturally occurring Crohn’s-like disease, they may be suitable to be used for studying the mechanism underlying VH in common intestinal disorders of large animals. In the present study, 60 healthy goats of either sex were equally divided into a 2, 4, 6-trinitrobenzenesulfonic acid (TNBS) group and saline group. A volume of 1.2 ml of TNBS-ethanol solution (30 mg TNBS in 40 % ethanol) or an equal volume of isotonic saline was injected into the wall of the terminal ileum through laparotomy. The severity of the developing ileitis was determined according to macro- and microscopic pathologic scores and the levels of myeloperoxidase, interleukin-1β, interleukin-6 and tumor necrosis factor-α, and VH was evaluated with visceromotor responses (VMR) to colorectal distension on days 3, 7, 14, 21 and 28. VMRs were assessed with a continuous ramp distention mode with 6 s for each pressure (20, 40, 60, 80 and 100 mmHg).
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机译：背景技术内脏超敏反应（VH）是许多胃肠道疾病（例如人和动物的炎症性肠病（IBD））的常见病状。大多数研究通常会诱发克罗恩氏病/结肠炎，以研究小型实验动物中的VH。尽管农场动物通常患有IBD，但迄今为止尚未对其VH进行调查。由于山羊可能患有约翰氏病（一种自然发生的克罗恩病），因此它们可能适合用于研究大型动物常见肠道疾病中VH的潜在机制。在本研究中，将60只雌雄同体的山羊均分为2组，4组，6组-三硝基苯磺酸（TNBS）组和生理盐水组。通过剖腹术将1.2 ml TNBS-乙醇溶液（30 mg TNBS溶于40％乙醇）或等体积等渗盐水注入回肠末端壁。根据宏观和微观病理学评分以及髓过氧化物酶，白介素-1β，白介素-6和肿瘤坏死因子-α的水平，确定发展中的回肠炎的严重性，并通过对大肠扩张的内脏动力反应（VMR）评估VH。第3、7、14、21和28天。VMR采用连续坡道扩张模式评估，每种压力（20、40、60、80和100 mmHg）为6s。
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