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Evaluation of Dual-Color Fluorescence In Situ Hybridization With Peptide Nucleic Acid Probes for the Detection of Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria in Clinical Specimens

机译：肽核酸探针双色荧光原位杂交在临床标本中检测结核分枝杆菌和非结核分枝杆菌的评价

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BackgroundPeptide nucleic acid (PNA) probes are artificial DNA analogues with a hydrophobic nature that can penetrate the mycobacterial cell wall. We evaluated a FISH method for simultaneous detection and identification of Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM) in clinical respiratory specimens using differentially labeled PNA probes.

机译：背景肽核酸（PNA）探针是具有疏水性质的人造DNA类似物，可以穿透分枝杆菌细胞壁。我们使用差异标记的PNA探针评估了FISH方法，用于同时检测和鉴定临床呼吸道标本中的结核分枝杆菌（MTB）和非结核分枝杆菌（NTM）。

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期刊名称 Annals of Laboratory Medicine
作者
Namhee Kim; Seung Hee Lee; Jongyoun Yi; Chulhun L. Chang;
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作者单位

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年(卷),期 2015(35),5
年度 2015
页码 500–505
总页数 6
原文格式 PDF
正文语种
中图分类情报学;
关键词
Peptide nucleic acids Fluorescence in situ hybridization Mycobacterium tuberculosis Non-tuberculous mycobacteria;

机译：肽核酸;荧光原位杂交;结核分枝杆菌;非结核分枝杆菌;

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专利

1. Evaluation of Dual-Color Fluorescence In Situ Hybridization With Peptide Nucleic Acid Probes for the Detection of Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria in Clinical Specimens [J] . Annals of laboratory medicine. . 2015,第5期

机译：肽核酸探针双色荧光原位杂交在临床标本中检测结核分枝杆菌和非结核分枝杆菌的评估
2. Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes for Rapid Detection of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis in Potable-Water Biofilms [J] . Markku J. Lehtola, Eila Torvinen, Ilkka T. Miettinen, Applied and Environmental Microbiology . 2006,第1期

机译：使用肽核酸探针的荧光原位杂交技术，用于快速检测鸟分枝杆菌亚种。鸟和分枝杆菌亚种。饮用水生物膜中的副结核病
3. Evaluation of Peptide Nucleic Acid-Fluorescence In Situ Hybridization for Identification of Clinically Relevant Mycobacteria in Clinical Specimens and Tissue Sections [J] . Michael Lefmann, Birgitta Schweickert, Petra Buchholz, Journal of Clinical Microbiology . 2006,第10期

机译：评估多肽核酸-荧光原位杂交技术在临床标本和组织切片中鉴定临床相关分枝杆菌的能力
4. EXPEDITIOUS IDENTIFICATION AND QUANTIFICATION OF MYCOBACTERIA SPECIES IN METALWORKING FLUIDS USING PEPTIDE NUCLEIC ACID PROBES [C] . Steven J. Skerlos, Laura A. Skerlos, Carlos A. Aguilar, Conference on Transactions of the North American Manufacturing Research Institution of SME May 20-23, 2003 Hamilton, Ontario, Canada . 2003

机译：使用肽核酸探针对金属加工液中的菌种进行快速鉴定和定量
5. Synthetic studies on peptide nucleic acids: 1. Toward a circular bis-peptide nucleic acid. 2. Sequence and linker effects in bis-PNA hybridization to DNA. [D] . Tse, Joseph. 2004

机译：肽核酸的合成研究：1.形成环状双肽核酸。 2. bis-PNA与DNA杂交中的序列和接头效应。
6. Evaluation of a Fluorescence In Situ Hybridization Assay for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Lowenstein-Jensen and Mycobacteria Growth Indicator Tube Cultures Using Peptide Nucleic Acid Probes [O] . Poonpilas Hongmanee, Henrik Stender, Ole F. Rasmussen 2001

机译：评估荧光原位杂交测定法在结核性和非结核性分枝杆菌的涂片中的应用低聚核苷酸和分枝杆菌生长指示剂的管核酸肽核酸探针。
7. Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes for Rapid Detection of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis in Potable-Water Biofilms [O] . Lehtola, Markku J., Torvinen, Eila, Miettinen, Ilkka T., 2006

机译：使用肽核酸探针的荧光原位杂交技术，用于快速检测鸟分枝杆菌亚种。鸟和分枝杆菌亚种。饮用水生物膜中的副结核病
8. Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): A Method for Bacterial Small RNA Detection [R] . Robertson, K. L., Vora, G. J. 2012

机译：锁核酸流式细胞仪 - 荧光原位杂交（LNa flow-FIsH）：一种细菌小RNa检测方法

Evaluation of Dual-Color Fluorescence In Situ Hybridization With Peptide Nucleic Acid Probes for the Detection of Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria in Clinical Specimens

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