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Composition of the Landfill Microbial Community as Determined by Application of Domain- and Group-Specific 16S and 18S rRNA-Targeted Oligonucleotide Probes

机译:垃圾填埋场微生物群落的组成通过域和组特定的16S和18S rRNA靶向寡核苷酸探针的应用确定。

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摘要

The microbial community composition of colonized cotton and leachate samples from a landfill was quantified using small subunit (SSU) rRNA probes (quantitative rRNA hybridization). Relative quantification of bacteria, eukaryotes, and archaea revealed variations in the landfill microbial community between samples from different areas of the landfill site and indicated the presence of potentially novel archaea. Anaerobic fungi were quantified in rumen fluid samples but were not sufficiently abundant for direct detection in the landfill samples.
机译:使用小亚基(SSU)rRNA探针(定量rRNA杂交)对来自垃圾填埋场的定植的棉花和渗滤液样品的微生物群落组成进行了定量。细菌,真核生物和古细菌的相对定量揭示了垃圾填埋场不同区域的样本之间垃圾掩埋微生物群落的变化,并表明存在潜在的新型古细菌。在瘤胃液样品中对厌氧真菌进行了定量,但其含量不足以直接在垃圾填埋场中进行检测。

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