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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >A Change in a Single Midgut Receptor in the Diamondback Moth (Plutella xylostella) Is Only in Part Responsible for Field Resistance to Bacillus thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai
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A Change in a Single Midgut Receptor in the Diamondback Moth (Plutella xylostella) Is Only in Part Responsible for Field Resistance to Bacillus thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai

机译：小菜蛾（Plutella xylostella）中单个中肠受体的变化仅部分负责苏云金芽孢杆菌亚种的田间抗性。库尔斯塔基和苏云金芽孢杆菌亚种。相泽

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A population (SERD3) of the diamondback moth (Plutella xylostella L.) with field-evolved resistance to Bacillus thuringiensis subsp. kurstaki HD-1 (Dipel) and B. thuringiensis subsp. aizawai (Florbac) was collected. Laboratory-based selection of two subpopulations of SERD3 with B. thuringiensis subsp. kurstaki (Btk-Sel) or B. thuringiensis subsp. aizawai (Bta-Sel) increased resistance to the selecting agent with little apparent cross-resistance. This result suggested the presence of independent resistance mechanisms. Reversal of resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai was observed in the unselected SERD3 subpopulation. Binding to midgut brush border membrane vesicles was examined for insecticidal crystal proteins specific to B. thuringiensis subsp. kurstaki (Cry1Ac), B. thuringiensis subsp. aizawai (Cry1Ca), or both (Cry1Aa and Cry1Ab). In the unselected SERD3 subpopulation (ca. 50- and 30-fold resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai), specific binding of Cry1Aa, Cry1Ac, and Cry1Ca was similar to that for a susceptible population (ROTH), but binding of Cry1Ab was minimal. The Btk-Sel (ca. 600-and 60-fold resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai) and Bta-Sel (ca. 80-and 300-fold resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai) subpopulations also showed reduced binding to Cry1Ab. Binding of Cry1Ca was not affected in the Bta-Sel subpopulation. The results suggest that reduced binding of Cry1Ab can partly explain resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai. However, the binding of Cry1Aa, Cry1Ac, and Cry1Ca and the lack of cross-resistance between the Btk-Sel and Bta-Sel subpopulations also suggest that additional resistance mechanisms are present.
机译：小菜蛾（Plutella xylostella L.）的种群（SERD3）具有对苏云金芽孢杆菌亚种的田间进化抗性。库尔斯塔基HD-1（Dipel）和苏云金芽孢杆菌亚种。收集了aizawai（Florbac）。基于实验室的苏云金芽孢杆菌亚种SERD3的两个亚群选择。库尔斯塔基（Btk-Sel）或苏云金芽孢杆菌亚种。 aizawai（Bta-Sel）增加了对选择剂的抗性，几乎没有明显的交叉抗性。该结果表明存在独立的抵抗机制。对苏云金芽孢杆菌亚种抗性的逆转。库尔斯塔基和苏云金芽孢杆菌亚种。在未选择的SERD3亚人群中观察到aizawai。检查对中肠刷缘膜囊的结合，以检测苏云金芽孢杆菌亚种特有的杀虫晶体蛋白。库尔斯塔基（Cry1Ac），苏云金芽孢杆菌亚种。 aizawai（Cry1Ca），或两者（Cry1Aa和Cry1Ab）。在未选择的SERD3亚群中（对苏云金芽孢杆菌kurstaki亚种和苏云金芽孢杆菌亚种aizawai的抗性约为50倍和30倍），Cry1Aa，Cry1Ac和Cry1Ca的特异性结合与易感人群（ROTH）相似），但Cry1Ab的结合极少。 Btk-Sel（对苏云金芽孢杆菌库尔斯塔克亚种和苏云金芽胞杆菌亚扎怀亚种的抗性约600和60倍）和Bta-Sel（对苏云金芽孢杆菌库尔斯塔克亚种抗性约80和300倍）和苏云金芽孢杆菌亚种（Aizawai）亚群也显示出与Cry1Ab的结合减少。在Bta-Sel亚群中，Cry1Ca的结合不受影响。结果表明，降低的Cry1Ab结合可以部分解释对苏云金芽孢杆菌亚种的抗性。库尔斯塔基和苏云金芽孢杆菌亚种。相泽但是，Cry1Aa，Cry1Ac和Cry1Ca的结合以及Btk-Sel和Bta-Sel亚群之间缺乏交叉抗性也表明存在其他抗性机制。

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