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Market Assessment and Product Evaluation of Probiotic Containing Dietary Supplements Available in Bangladesh Market

机译:孟加拉国市场上含益生菌膳食补充剂的市场评估和产品评估

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摘要

Probiotics containing food supplements available in Bangladesh market were identified and collected for assessment. To assess their label claim, they were resuspended into sterile distilled water. Then, series dilutions of each sample solution were prepared and immediately plated out, in duplicate, into De Man Rogosa Sharpe (MRS) agar. These plates were then incubated at 37°C for 48 hours and colonies were counted. Viable cell numbers stated on the labels were compared with actual viable cell numbers. To assess the viability of the probiotics included in the products, probiotic strains were isolated from each of the four products and screened for inhibitory activity against six indicator strains. It was surprisingly found that although the viable cell numbers of all supplements were three to four log cycles lower than label claim of the products, however, this problem did not affect the inhibitory activity of the probiotic strains against indicator strains according to in vitro assessment. Legislation and regulation regarding prebiotic-probiotic containing products should be built up in Bangladesh to ensure quality products supply to the consumers. Moreover, manufacturers of probiotic containing products should take the responsibility for providing the consumer with scientifically and legally correct information.
机译:确定并收集了孟加拉国市场上含有食品补充剂的益生菌,以进行评估。为了评估其标签要求,将它们重新悬浮在无菌蒸馏水中。然后,制备每种样品溶液的系列稀释液,并立即一式两份地铺板到De Man Rogosa Sharpe(MRS)琼脂中。然后将这些板在37℃下孵育48小时,并计数菌落。将标签上注明的活细胞数与实际活细胞数进行比较。为了评估产品中所含益生菌的生存能力,从四种产品中的每一种中分离了益生菌菌株,并筛选了对六种指示菌株的抑制活性。令人惊讶地发现,尽管所有补品的活细胞数比产品的标签要求少三至四个对数周期,但是根据体外评估,该问题并未影响益生菌菌株对指示菌株的抑制活性。孟加拉国应建立有关含益生菌和益生菌产品的法律法规,以确保向消费者提供优质的产品。此外,含益生菌产品的制造商应负责向消费者提供科学和法律上正确的信息。

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