Nickel quercetinase a promiscuous metalloenzyme: metal incorporation and metal ligand substitution studies

摘要

BackgroundQuercetinases are metal-dependent dioxygenases of the cupin superfamily. While fungal quercetinases are copper proteins, recombinant Streptomyces quercetinase (QueD) was previously described to be capable of incorporating Ni²⁺ and some other divalent metal ions. This raises the questions of which factors determine metal selection, and which metal ion is physiologically relevant.