Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

摘要

BackgroundIn real-time PCR, it is necessary to consider the efficiency of amplification (EA) of amplicons in order to determine initial target levels properly. EAs can be deduced from standard curves, but these involve extra effort and cost and may yield invalid EAs. Alternatively, EA can be extracted from individual fluorescence curves. Unfortunately, this is not reliable enough.