Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients

摘要

class="kwd-title">Аbbreviation: AFM, atomic force microscopy; MN, monocytes; MOC, mononuclear cells; MPh, macrophages; MUC, multinucleated cells; PHAs, polyhydroxyalcanoates; P(3HB), poly-3-hydroxybutyrate; P(3HB/3HV), copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate; P(3HB/4HB), copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate; P(3HB/3HV/3HHx), copolymers of 3-hydroxybutyrate, 3-hydroxyvalerate and 3-hydroxyhexanoate; P(3HB/3HV/4HB/3HHx), copolymers of 3-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate and 3-hydroxyhexanoate; SEM, scanning electron microscopy class="kwd-title">Keywords: Monocytes, Macrophages, Cell morphology, Polyhydroxyalkanoates, Atherosclerosis, Intravascular stenting class="head no_bottom_margin" id="abs0015title">AbstractThe morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the microano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices.The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the microano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients.