首页> 美国卫生研究院文献>Journal of Visualized Experiments : JoVE >Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography
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Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography

机译:使用延时微电影照相技术记录黄黏菌生物膜中的多细胞行为。

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摘要

A swarm of the δ-proteobacterium Myxococcus xanthus contains millions of cells that act as a collective, coordinating movement through a series of signals to create complex, dynamic patterns as a response to environmental cues. These patterns are self-organizing and emergent; they cannot be predicted by observing the behavior of the individual cells. Using a time-lapse microcinematography tracking assay, we identified a distinct emergent pattern in M. xanthus called chemotaxis, defined as the directed movement of a swarm up a nutrient gradient toward its source 1.In order to efficiently characterize chemotaxis via time-lapse microcinematography, we developed a highly modifiable plate complex (Figure 1) and constructed a cluster of 8 microscopes (Figure 2), each capable of capturing time-lapse videos. The assay is rigorous enough to allow consistent replication of quantifiable data, and the resulting videos allow us to observe and track subtle changes in swarm behavior. Once captured, the videos are transferred to an analysis/storage computer with enough memory to process and store thousands of videos. The flexibility of this setup has proven useful to several members of the M. xanthus community.
机译:δ-变形杆菌Xanthus菌群包含数百万个细胞,这些细胞作为一个集体,通过一系列信号协调运动,从而创建复杂的动态模式以响应环境提示。这些模式是自组织的和紧急的;无法通过观察单个细胞的行为来预测它们。使用延时微电影照相法跟踪分析,我们在花椒中鉴定了一种独特的出芽模式,称为趋化性,定义为一群营养素向营养源 1 向上的定向运动。通过延时微电影照相法表征趋化性,我们开发了高度可修饰的平板复合物(图1),并构建了由8个显微镜组成的群集(图2),每个显微镜都能够捕获延时视频。该测定非常严格,可以一致地复制可量化的数据,并且产生的视频使我们能够观察和跟踪群体行为的细微变化。捕获后,视频将传输到具有足够内存以处理和存储数千个视频的分析/存储计算机。实践证明,此设置的灵活性对M. xanthus社区的一些成员很有用。

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