Plasmalemma-enriched vesicles were isolated from cotton roots (Gossypium hirsutum L. cv Acala San Jose 2) and from germinating radish seeds (Raphanus sativa L. cv Tondo Rosso Quarantino). When 100 millimolar ascorbate was added to the grinding medium, the addition of ferricyanide to either preparation led to an inside positive membrane potential as measured by the accumulation of thiocyanate. It is suggested that electrons from ascorbate were being transported electrogenically across the membrane to ferricyanide, resulting in an accumulation of protons within the vesicle. The redox activity of the vesicles has some similarities to that occurring in intact cells, thus providing a simpler system to study the components and effects of transmembrane electron transport.
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机译：从棉根（Gossypium hirsutum L.cv Acala San Jose 2）和发芽的萝卜种子（Raphanus sativa L.cv Tondo Rosso Quarantino）中分离富含血浆的丝虫。当将100毫摩尔抗坏血酸酯添加到研磨介质中时，将铁氰化物添加到任一种制剂中都导致内部正膜电位，如通过硫氰酸盐的积累所测量的。这表明来自抗坏血酸盐的电子被电子地跨膜传输到铁氰化物，导致质子在囊泡中积累。囊泡的氧化还原活性与完整细胞中的氧化还原活性有些相似，因此提供了一个更简单的系统来研究跨膜电子传输的成分和作用。
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