The levels of chloroplast DNA in a cultured photoautotrophic soybean (Glycine max [L.] Merr. v Corsoy) cell line were determined by molecular hybridization. The cells were also grown photomixotrophically and heterotrophically as suspension cultures and the level of plastid DNA was found to be constant at approximately 26% of the total cellular DNA in all three growth modes. By comparison, total cellular DNA extracted from plants of the same variety used as the explant source for the cultured cells contained 12.3 to 18.9% (leaves and seeds) and 6.1 to 8.9% (roots) plastid DNA.
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机译:通过分子杂交测定培养的光合自养大豆(Glycine max [L.] Merr。v Corsoy)细胞系中叶绿体DNA的水平。还使细胞光混合营养和异营养生长为悬浮培养物,并且在所有三种生长模式下,质体DNA的水平恒定在总细胞DNA的约26%。相比之下,从相同品种的植物中提取的总细胞DNA用作培养细胞的外植体来源,含有12.3至18.9%(叶和种子)和6.1至8.9%(根)的质体DNA。
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