Adult rat cardiac myocytes in culture: ‘Second-floor’ cells and coculture experiments

摘要

OBJECTIVETo compare adult rat cardiomyocytes in primary culture for up to 28 days with those in primary culture for 10 days plus up to 18 days in a coculture (CC) system. The phenomenon of ‘second-floor’ cells in primary cultures and the behaviour of CC myocytes were studied over varying times with regard to protein content and attachment to underlying cells.