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Parameters for Successful Parental RNAi as An Insect Pest Management Tool in Western Corn Rootworm Diabrotica virgifera virgifera

机译:成功的亲本RNAi作为西部玉米根虫中的病虫害防治工具的参数

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摘要

Parental RNAi (pRNAi) is an RNA interference response where the gene knockdown phenotype is observed in the progeny of the treated organism. pRNAi has been demonstrated in female western corn rootworms (WCR) via diet applications and has been described as a potential approach for rootworm pest management. However, it is not clear if plant-expressed pRNAi can provide effective control of next generation WCR larvae in the field. In this study, we evaluated parameters required to generate a successful pRNAi response in WCR for the genes brahma and hunchback. The parameters tested included a concentration response, duration of the dsRNA exposure, timing of the dsRNA exposure with respect to the mating status in WCR females, and the effects of pRNAi on males. Results indicate that all of the above parameters affect the strength of pRNAi phenotype in females. Results are interpreted in terms of how this technology will perform in the field and the potential role for pRNAi in pest and resistance management strategies. More broadly, the described approaches enable examination of the dynamics of RNAi response in insects beyond pRNAi and crop pests.
机译:亲本RNAi(pRNAi)是一种RNA干扰反应,其中在被治疗有机体的后代中观察到基因敲低表型。 pRNAi已通过饮食应用在雌性西部玉米根虫(WCR)中得到证明,并被描述为用于根虫害虫管理的一种潜在方法。然而,尚不清楚植物表达的pRNAi是否可以在田间有效控制下一代WCR幼虫。在这项研究中,我们评估了梵文和驼背基因在WCR中产生成功的pRNAi反应所需的参数。测试的参数包括浓度响应,dsRNA暴露持续时间,dsRNA暴露时间(相对于WCR雌性的交配状态)以及pRNAi对雄性的影响。结果表明上述所有参数均会影响女性的pRNAi表型强度。根据该技术在野外的表现以及pRNAi在有害生物和抗性管理策略中的潜在作用来解释结果。更广泛地说,所描述的方法使得能够检查除pRNAi和农作物害虫之外的昆虫中RNAi应答的动力学。

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