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A Highlights from MBoC Selection: Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy

机译:MBoC选择的亮点:通过点阵光片显微镜成像的分裂细胞的膜动力学

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摘要

Membrane remodeling is an essential part of transferring components to and from the cell surface and membrane-bound organelles and for changes in cell shape, which are particularly critical during cell division. Earlier analyses, based on classical optical live-cell imaging and mostly restricted by technical necessity to the attached bottom surface, showed persistent formation of endocytic clathrin pits and vesicles during mitosis. Taking advantage of the resolution, speed, and noninvasive illumination of the newly developed lattice light-sheet fluorescence microscope, we reexamined their assembly dynamics over the entire cell surface and found that clathrin pits form at a lower rate during late mitosis. Full-cell imaging measurements of cell surface area and volume throughout the cell cycle of single cells in culture and in zebrafish embryos showed that the total surface increased rapidly during the transition from telophase to cytokinesis, whereas cell volume increased slightly in metaphase and was relatively constant during cytokinesis. These applications demonstrate the advantage of lattice light-sheet microscopy and enable a new standard for imaging membrane dynamics in single cells and multicellular assemblies.
机译:膜重塑是将成分转移至细胞表面和细胞膜结合的细胞器以及从细胞表面和细胞膜转移细胞膜以及细胞形状变化的重要组成部分,这在细胞分裂过程中尤其重要。较早的分析基于经典的光学活细胞成像,并且在很大程度上受技术要求限制在附着的底表面上,显示在有丝分裂期间持续形成内吞网格蛋白坑和囊泡。利用新开发的点阵光片荧光显微镜的分辨率,速度和无创照明,我们重新检查了它们在整个细胞表面上的装配动力学,发现网格蛋白坑在有丝分裂后期形成的速率较低。对培养中和斑马鱼胚胎中单个细胞整个细胞周期的细胞表面积和体积进行全细胞成像测量,结果表明,从末期到胞质分裂的过渡过程中,总表面迅速增加,而中期则细胞体积略有增加,并且相对恒定在胞质分裂过程中。这些应用证明了点阵光片显微镜的优势,并为成像单细胞和多细胞组件中的膜动力学提供了新的标准。

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