幽门螺杆菌感染及根除后胃黏膜CD4+CD25+调节性T细胞表达的变化

摘要

目的 观察幽门螺杆菌(helicobacter pylori, Hp)感染及根除后CD4+CD25+调节性T细胞的表达变化,以探讨Hp长期定植在胃黏膜引起慢性持续性感染的原因.方法 采用免疫组化法分析45例Hp阳性患者(研究组)Hp清除前后及35例Hp阴性患者(对照组)胃黏膜中的CD4+CD25+调节性T细胞的表达变化. 结果 Hp阳性患者局部胃黏膜中的CD4+CD25+调节性T细胞明显增多,占5.012%,明显高于Hp阴性患者胃黏膜中的CD4+CD25+调节性T细胞,占0.739%,差异有统计学意义 (P＜0.01).根除Hp治疗后CD4+CD25+调节性T细胞明显减少,占0.551%,明显低于清除前,差异有统计学意义(P＜0.01). 结论 Hp感染后可能通过上调CD4+CD25+调节性T细胞的表达,抑制Hp引起的特异性T细胞反应,造成Hp的长期定植.根除Hp后人胃黏膜内CD4+CD25+调节性T细胞明显减少可能是Hp被清除后,该类细胞凋亡或者移出胃黏膜所致.%Objective To investigate the expression of CD4+CD25+ regulatory T cells in patients with Helicobacter pylori (H.pylori) infection before and after eradication.Methods Biopsies of gastric mucosa were performed in 45 H.pylori- positive patients (test group) before and after H.pylori eradication and 35 H.pylori- negative adults (control group).Immunohistochemical staining was used to semi- quantify the number of CD4+CD25+T cells in gastric mucosa.Results The ratio of CD25+ T cell in CD4+ T cells accounted for 0.739％ in H.pylori- negative individuals, while it was 5.012％ in H.pylori- positive patients (P＜0.01).After eradication of H.pylori the ratio of CD25+ T cell in CD4+T cells declined to 0.551％ (P＜0.01).Conclusion The results indicate that H.pylori infection may up- regulate CD4+ CD25+ regulatory T cells, suppressing the expression of H.pylori- specific T cell response and resulting in long- term H.pylori colonization.