六味地黄丸对甲状腺素所致甲亢大鼠脂肪组织UCP2、骨骼肌UCP3mRNA表达的影响

摘要

目的：探讨六味地黄丸对甲状腺素所致甲亢大鼠脂肪组织UCP2、骨骼肌UCP3mRNA表达的影响.方法：雄性SD大鼠48只,随机分为空白组、甲状腺素甲亢模型组、甲状腺素+低剂量六味地黄丸组和甲状腺素+高剂量六味地黄丸组,每组12只.利用RT-PCR测定各组大鼠脂肪组织UCP2与骨骼肌UCP3mRNA表达,并检测各组大鼠血清T3、T4,红细胞Na+-K+-ATP酶活力,肝脏MDA含量和SOD活力.结果：与甲状腺素甲亢模型组相比,甲状腺素+高剂量六味地黄丸组大鼠脂肪组织UCP2和骨骼肌UCP3mRNA表达水平显著下调(P<0.05),红细胞Na+-K+-ATP酶活力降低(P<0.05),血清T3、T4以及肝组织MDA含量显著降低(P<0.01),肝脏SOD活力显著增加(P<0.01)；甲状腺素+低剂量六味地黄丸组甲亢大鼠脂肪组织UCP2表达水平下调,但差异无统计学意义(P>0.05),骨骼肌UCP3mRNA表达水平显著下调(P<0.05),血清T3(P<0.05)、T4以及肝组织MDA含量显著降低(P<0.01),红细胞Na+-K+-ATP酶活力降低,但差异无统计学意义(P>0.05),肝脏SOD活力显著增加(P<0.01).结论：六味地黄丸可以下调甲状腺素所致甲亢大鼠脂肪组织UCP2和骨骼肌UCP3mRNA表达.%　　Objective: To observe the effects of Liuwei Dihuang pills on the expression of UCP2 mRNA of adi⁃pose tissue and UCP3 mRNA of skeletal muscle in hyperthyroid rats induced by Thyroxine. Methods:For⁃ty-eight male SD rats were randomly divided into blank control group,hyperthyroid model of thyroxine group,thy⁃roxine plus low-dose Liuwei Dihuang pills group, and thyroxine plus high-dose Liuwei Dihuang pills group, 12 rats in each. The expression of UCP2 mRNA of adipose tissue and UCP3 mRNA of skeletal muscle in rats of all groups were detected by RT-PCR. The serum T3 and T4 and Na+-K+-ATPase activity of red blood cells, MDA content and SOD activity of liver were measured. Results: Compared with the hyperthyroid model of thyrox⁃ine group, the thyroxine plus high-dose Liuwei Dihuang pills group had reduced expression of UCP2 mRNA and UCP3 mRNA(P<0.05),decreased Na+-K+-ATPase activity(P<0.05),lower serum T3 and T4 and liver MDA content(P<0.01), and increased SOD activity(P<0.01); thyroxine plus low-dose Liuwei Dihuang pills group al⁃so had reduced expression of UCP2 mRNA in number (P>0.05)and down-regulated UCP3 mRNA with a signifi⁃cant difference(P<0.05),lower serum T3 and T4 content and liver MDA content(P<0.05),decreased Na +-K +-ATPase activity in number (P>0.05), and increased SOD activity(P<0.01). Conclusion: Liuwei Di⁃huang Pills can down-regulate the expression of UCP2 mRNA of adipose tissue and UCP3 mRNA of skeletal muscle in hyperthyroid rats induced by thyroxine.