高脂肪饮食对大鼠青春期启动影响的实验研究

摘要

目的：观察高脂肪饮食对大鼠青春期启动的影响。方法20只雌性SD大鼠按配对设计的分组原则，分为标准组、高脂组，各20只。实验第1天起标准组大鼠喂养标准饲料，高脂组大鼠喂养高脂肪饲料。每天观察大鼠阴道口开放情况，当高脂组大鼠全部出现阴道口开放，即于当日下午处死全部大鼠。观察两组大鼠阴道口开放时间（VO）及第一性周期时间（D1），计算子宫、卵巢指数，采用放射免疫法检测血清黄体生成素（LH）的含量。结果与标准组比较，高脂组阴道口开放时间（VO）（33.2±1.87）天，第一动情间期时间（D1）（35.7±1.16）天，均较标准组的（36.4±0.96）天、（37.5±0.53）天明显提前（P<0.01），LH含量高脂组（6.275±0.422）mIU/mL，标准组（4.979±0.587）mIU/mL，两组比较，差异有统计学意义（P<0.05）。结论高脂肪饮食可促使大鼠青春期启动提前。%Objective To investigate the effect of high fat diet on the onset of puberty of rats. Methods Twen-ty female SD rats were randomized into standard group and high-fat group by paired design, 10 for each group. From the first day of the experiment, rats in standard group were fed with standard diet, rats in high-fat group were fed with high fat diet. When all 10 rats in high-fat group had vaginal opening (VO), then they were sacri-ficed on the afternoon of that day. The times of VO and first cycle(D1) were observed, the uterus index and ovar-ian index were calculated, and the content of serum luteinizing hormone (LH) was detected by radioimmunoassay. Results Compared with standard group, VO and D1 was significantly earlier in high-fat group (VO:33.2±1.87d vs 36.4±0.96d;D1:35.7±1.16d vs 37.5±0.53d;all P<0.01),and the serum LH were statistically increased in high-fat group(6.275±0.422mIU/mL vs 4.979±0.587mIU/mL,P<0.05). Conclusion The high fat diet can induce early puber-ty starts in rats.