大蒜素对慢性铁过负荷大鼠氧化应激的保护作用

摘要

目的探讨大蒜素对慢性铁负荷大鼠抗氧化功能的影响及可能机制.方法36只SD大鼠按体重随机分为6组,即正常对照组(饲喂基础饲料)、慢性铁负荷模型组(饲喂高铁饲料)、正常+大蒜素40mg/kg组、慢性铁负荷+大蒜素30、40、60mg/kg组,每组6只.持续饲喂6周后,取血清、肝、结肠和肾组织测定铁含量、总铁结合力、丙二醛(MDA)含量及总超氧化物歧化酶(T-SOD)活性.结果与正常对照组比较,模型组血清铁、肝和结肠组织铁、MDA含量及肝组织T-SOD活性均显著升高(P<0.05,P<0.01),血清总铁结合力、结肠组织T-SOD活性均显著降低(P<0.01)；与模型组比较,各剂量大蒜素组血清、结肠组织铁、肝组织MDA含量及中、高剂量大蒜素组肝组织铁含量均显著降低(P<0.05),血清总铁结合力、结肠和肾组织T-SOD活性均显著升高(P<0.05).结论大蒜素能增强大鼠机体的抗氧化能力,对铁过负荷所致的氧化应激具有一定的保护作用.%Objective To explore the protective effects of allicin on oxidative stress and its possible mechanism. Methods 36 SD rats were randomly divided into 6 groups:control group (basal diet, 50 mg·kg-1 Fe), iron group (high iron diet, 1000 mg·kg-1 Fe), allicin group (basal diet, ig given allicin 40 mg·kg-1), iron+low, medium and high allicin groups (high iron diet, ig given allicin 30, 40 and 60 mg·kg-1), once daily for 6 weeks. Levels of serum and tissues iron status, total iron-binding capacity (TIBC), MDA and T-SOD were determined. Results Compared with control group, the contents of serum iron status, liver and colon tissues iron status, MDA, and liver tissues T-SOD in model group were all increased significantly (P<0.05, P<0.01), while serum TIBC, colon tissues T-SOD were all decreased significantly (P<0.01);Compared with model group, the contents of serum and colon tissues iron status, liver tissues MDA in each dose group, and liver tissues iron status in medium and high allicin groups were all decreased significantly (P<0.05), while serum TIBC, colon and renal tissues T-SOD were all increased significantly (P<0.05). Conclusion Allicin can increase antioxidative effect and protect against oxidative stress in rats induced by iron overload.