补肾护肝法对大鼠酒精性脂肪肝的保护作用

摘要

Objective To observe the kidney prescription in the serum enzyme activity of alcoholic fatty liver model rats and the effect on the liver function,to explore the protection effect of the prescription of alcoholic fatty liver.Methods56 degrees hongxing spirit erguotou wine ig(10 ml/kg,2 times/d,for 8 weeks)method to establish the rat model with alcoholic fatty liver. After 8 weeks , the rats model were divided into control group and treatment control group( with Haiwangjinzun drug),kidney prescription treatment groups(prepared rhizome of rehmannia,medicinal indianmulberry root,a fairy spleen)high,medium and low dose group,respectively with(3.0g/ml)10ml/(kg·d)、(1.5g/ml)10ml/(kg·d)、(0.75g/ml)10ml/(kg·d)lavage treatment;Model control group with the same amount of distilled water,for 4 weeks in a row,at the same time establish normal control group.12 h after the treatment,fasting,liver detection index,liver portal vein blood serum detection,serum gamma-glutamine transferase(γ-GT),aspertate aminotransferase(AST),alanine aminotransferase(ALT)activity.Results For the liver function index in the model group,gamma GT,ALT and AST activity were higher than normal control group significantly(P<0.05),body quality was significantly lower(P<0.05); in the high,medium and low dose of Chinese medicine group,western medicine group liver index,γ-GT,ALT and AST activity were significantly lower in the model group(P<0.05),body quality significantly increased(P<0.05).Conclusion The kidney prescription of Chinese traditional medicine can reduceγ-GT,ALT,AST,reduce the accumulation of hepatic fat,reduce the damage in liver cells, thus having protective effect on the alcoholic fatty liver.%目的：观察补肾方对酒精性脂肪肝模型大鼠血清酶活力及肝脏指数的影响，探讨本处方对酒精性脂肪肝的保护作用。方法以56°红星二锅头酒（10ml/kg，2次/d，连续8周）的方法建立酒精性脂肪肝大鼠模型。8周后将造模成功大鼠分为模型对照组、海王对照组（金樽海王片）、补肾方（熟地、巴戟天、仙灵脾）高、中、低剂量组，分别以（3.0g/ml）10ml/（kg·d）、（1.5g/ml）10ml/（kg·d）、（0.75g/ml）10ml/（kg·d）灌胃处理；模型对照组用等量的蒸馏水，连续给药4周，同期设正常对照组。给药结束后，禁食12 h，取肝脏检测肝脏指数，门静脉取血检测血清γ-谷氨酰转移酶（γ-GT）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）活力。结果与空白组比较，模型组肝脏指数、γ-GT、ALT及AST 活力显著升高（P<0.05），体质量显著降低（P<0.05）；中药高、中、低剂量组、海王组肝脏指数、γ-GT、ALT及AST 活力较模型组显著性降低（P<0.05），体质量显著性上升（P<0.05）。结论补肾方能降低γ-GT、ALT、AST活力，减少肝脏脂肪的蓄积，降低对肝细胞的损伤，对酒精性脂肪肝确有保护作用。