[Objective] This project aims to identify the strains that can degrade the low rank coal from the collected samples of coal,rotten wood and soil samples in Xinjiang and study the transformation ability of bacteria to the low rank coal.[Method]The strains were isolated and purified with beef extract peptone culture medium;Through the preliminary screening,the CZAPECK DOX-medium selected became a dark brown or black strain.Then the liquid medium was used to determine the dissolution rate.The phylogenetic tree of 16S rDNA gene was thus constructed for biologically identifying the strains.[Result] 191 strains of bacteria were isolated from the samples collected,that was 25 strains,61 strains and 105 strains were isolated from the samples of coal,rotten wood and soil,which accounted for 13％,32％,54％ of the total,respectively.Through the preliminary screening,28 strains with the function of solubilization of coal were selected,among which,the dissolution rates of NH-6,NF-1,NH-3,YF-3 and NG-4 were 35.06％,34.72％,25.23％,28.03％ and 35.89％,respectively.After preliminary identification,NG-4,NF-1 and YF-3 all belonged to Bacillus genus (Bacillus) and NH-3,NH-6 belonged to fake single spore strains (Pseudomonas).[Contusion]The selected strains of Bacillus sp.(Bacillus) and Pseudomonas sp.(Pseudomonas) have a higher degradation ability to Xinjiang low rank coal,which supplement the low rank coal degradation bacteria resources in Xinjiang.%[目的]从采集的煤、腐木和土壤样品中分离、鉴定出能够高效降解新疆低阶煤的菌株,研究细菌对新疆低阶煤的转化能力.[方法]用牛肉膏蛋白胨培养基分离、纯化菌株;通过初步筛选,选出发酵之后CZA-PECK DOX-medium培养基变成深褐色或者黑色的菌株,之后利用液体培养基对菌株的溶煤率进行测定;构建溶煤菌株的16S rDNA基因系统发育树并对菌株进行生物学鉴定.[结果]从采集的样品中共分离出191株细菌,从煤样品、腐木样品和土壤样品中分别分离出25、61和105株菌,分别占总数的13％、32％、54％.通过初步筛选,选出28株具有溶煤功能的菌株,其中NH-6、NF-1、NH-3、YF-3、NG-4的溶煤率分别为35.06％、34.72％、25.23％、28.03％、35.89％,对新疆低阶煤具有较好的降解能力.经过初步鉴定NG-4、NF-1、YF-3均属于芽孢杆菌属(Bacillus);NH-3、NH-6属于假单孢菌属(Pseudomonas).[结论]筛选得到的几株芽孢杆菌属(Bacillus)和假单孢菌属(Pseudomonas)的菌株对新疆低阶煤具有较高的降解能力,补充了新疆低阶煤降解菌资源.
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