cqvip:Background: Tape stripping is a commonmethod for investigating stratum corneu m (SC) physiology as well as bioavailability and bioequivalence of topical drugs . Objectives: To investigate the influence of procedures (anatomical site, press ure, pressure duration, tape removal rate) inherent in each stripping protocol o n changes in skin physiology. Methods: Tape stripping was performed using tapes on the forearm, forehead and back. On the forearm different pressures (165 and 3 30 g cm- 2), durations of pressure (2 and 10 s), and removal rate (slow and rap id removal) were used. Changes in skin physiology were evaluated by measurement of transepidermal water loss (TEWL) and hydration. Results: Asignificant influen ce of all parameters on the TEWL increase as a function of tape strip numberwas observed. The fastest increasewas demonstrated on the forehead, followed by the back and, lastly, the forearm. Rapid removal produced a protracted increase in c omparison with slow removal. Pressure for 10 s induced a faster increase in TEWL than 2 s pressure. Likewise, pressure at 330 g cm- 2 induced an earlier increa se than pressure at 165 g cm- 2. Skin hydration was not influenced by the varia bles tested. Conclusions: Tape stripping results are influenced dramatically by all investigated parameters. A dynamic SC stress test to investigate SC cohesion more closely is proposed based on the present observations.
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