家族性肌萎缩侧索硬化动物模型腰段脊髓中泛素表达特征

摘要

目的了解泛素在家族性肌萎缩侧索硬化动物模型中脊髓前角运动神经元变性、丢失过程中的表达特征；方法利用免疫组织化学、蛋白免疫印迹的方法观察脊髓前角运动神经元泛素表达；结果动物模型的病情进展过程中，腰髓前角运动神经元数量减少，泛素阳性蛋白聚集体逐渐增加，泛素的蛋白水平表达量无明显增加（P＞0.05）；结论家族性肌萎缩侧索硬化动物模型发病过程中蛋白的泛素化发生异常。%Objective To understand the role of ubiquitin in family amyotrophic lateral sclerosis animal model spinal cord degeneration.Methods Observed the expression of ubiquitin in spinal cord by immunohistochemistry,western blotting.Results As family amyotrophic lateral sclerosis animal model disease progress, the number of anterior horn neuron in lumbar spinal cord decreased ,ubiquitin positive aggregates increased.Conclusion The study suggests ubiquitination is abnormal in the disease progress of family amyotrophic lateral sclerosis animal model.