Coexpression of cholecystokinin-B/gastrin receptor and gastrin gene in human gastric tissues and gastric cancer cell line

摘要

AIM:To compare the expression patterns of cholecystokinin-B (CCK-B)/gastrin receptor genes in matched human gastric carcinoma and adjacent non-neoplastic mucosa of patients with gastric cancer,inflammatory gastric mucosa from patients with gastritis,normal stomachs from 2 autopsied patients and a gastric carcinoma cell line (SGC-7901), and to explore their relationship with progression to malignancy of human gastric carcinomas.METHODS:RT-PCR and sequencing were employed to detect the mRNA expression levels of CCK-B receptor and gastrin gene in specimens from 30 patients with gastric carcinoma and healthy bordering non-cancerous mucosa,10 gastritis patients and normal stomachs from 2 autopsied patients as well as SGC-7901.The results were semi-quantified by normalizing it to the mRNA level of β-actin gene using Lab Image software.The sequences were analyzed by BLAST program.RESULTS:CCK-B receptor transcripts were detected in all of human gastric tissues in this study, including normal,inflammatory and malignant tissues and SGC-7901. However,the expression levels of CCK-B receptor in normal gastric tissues were higher than those in other groups (P0.05).On the other hand,gastrin mRNA was detected in SGC-7901 and in specimens obtained from gastric cancer patients (22/30)but not in other gastric tissues,and its expression was highly correlated with the metastases of gastric cancer (P<0.05).CONCLUSION:Human gastric carcinomas and gastric cancer cell line SGC-7901 cells coexpress CCK-B receptor and gastrin mRNA. Gastrin/CCK-B receptor autocrine or paracrine pathway may possibly play an important role in the progression of gastric cancer.